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mini transblot cell wet transfer system  (Bio-Rad)


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    Structured Review

    Bio-Rad mini transblot cell wet transfer system
    Mini Transblot Cell Wet Transfer System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mini+transblot+cell+wet+transfer+system/mini+trans+blot+cell/pm36976213-162-90-97
    Average 90 stars, based on 1 article reviews
    mini transblot cell wet transfer system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid Shrimp.
    Article Snippet: .. For GST pull-down analysis, purified recombinant proteins (i.e., GST-PvBip, PvHMC, and GST) were incubated with Glutathione Sepharose 4B beads (Cat#17-0756, GE Healthcare, Boston, MA, USA) at 4 ◦C for 2 h. Next, samples were washed ten times with 0.01 M PBS (containing 1% triton) before being boiled with 5× loading buffer for 10 min. For SDS-PAGE and Western blot analyses, the samples prepared and boiled with 5× loading buffer, as described above, were separated on SDS-PAGE before being transferred onto PVDF membranes (Cat# R0NB30936, Millipore, Billerica, MA, USA) using a Mini TransBlot cell wet transfer system (Model#1658030, Bio-Rad, Richmond, CA, USA). ..

    Article Title: Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid Shrimp
    Article Snippet: .. For GST pull-down analysis, purified recombinant proteins (i.e., GST-PvBip, PvHMC, and GST) were incubated with Glutathione Sepharose 4B beads (Cat#17-0756, GE Healthcare, Boston, MA, USA) at 4 °C for 2 h. Next, samples were washed ten times with 0.01 M PBS (containing 1% triton) before being boiled with 5× loading buffer for 10 min. For SDS-PAGE and Western blot analyses, the samples prepared and boiled with 5× loading buffer, as described above, were separated on SDS-PAGE before being transferred onto PVDF membranes (Cat# R0NB30936, Millipore, Billerica, MA, USA) using a Mini TransBlot cell wet transfer system (Model#1658030, Bio-Rad, Richmond, CA, USA). ..

    Recombinant:

    Article Title: Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid Shrimp.
    Article Snippet: .. For GST pull-down analysis, purified recombinant proteins (i.e., GST-PvBip, PvHMC, and GST) were incubated with Glutathione Sepharose 4B beads (Cat#17-0756, GE Healthcare, Boston, MA, USA) at 4 ◦C for 2 h. Next, samples were washed ten times with 0.01 M PBS (containing 1% triton) before being boiled with 5× loading buffer for 10 min. For SDS-PAGE and Western blot analyses, the samples prepared and boiled with 5× loading buffer, as described above, were separated on SDS-PAGE before being transferred onto PVDF membranes (Cat# R0NB30936, Millipore, Billerica, MA, USA) using a Mini TransBlot cell wet transfer system (Model#1658030, Bio-Rad, Richmond, CA, USA). ..

    Article Title: Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid Shrimp
    Article Snippet: .. For GST pull-down analysis, purified recombinant proteins (i.e., GST-PvBip, PvHMC, and GST) were incubated with Glutathione Sepharose 4B beads (Cat#17-0756, GE Healthcare, Boston, MA, USA) at 4 °C for 2 h. Next, samples were washed ten times with 0.01 M PBS (containing 1% triton) before being boiled with 5× loading buffer for 10 min. For SDS-PAGE and Western blot analyses, the samples prepared and boiled with 5× loading buffer, as described above, were separated on SDS-PAGE before being transferred onto PVDF membranes (Cat# R0NB30936, Millipore, Billerica, MA, USA) using a Mini TransBlot cell wet transfer system (Model#1658030, Bio-Rad, Richmond, CA, USA). ..

    Incubation:

    Article Title: Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid Shrimp.
    Article Snippet: .. For GST pull-down analysis, purified recombinant proteins (i.e., GST-PvBip, PvHMC, and GST) were incubated with Glutathione Sepharose 4B beads (Cat#17-0756, GE Healthcare, Boston, MA, USA) at 4 ◦C for 2 h. Next, samples were washed ten times with 0.01 M PBS (containing 1% triton) before being boiled with 5× loading buffer for 10 min. For SDS-PAGE and Western blot analyses, the samples prepared and boiled with 5× loading buffer, as described above, were separated on SDS-PAGE before being transferred onto PVDF membranes (Cat# R0NB30936, Millipore, Billerica, MA, USA) using a Mini TransBlot cell wet transfer system (Model#1658030, Bio-Rad, Richmond, CA, USA). ..

    Article Title: Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid Shrimp
    Article Snippet: .. For GST pull-down analysis, purified recombinant proteins (i.e., GST-PvBip, PvHMC, and GST) were incubated with Glutathione Sepharose 4B beads (Cat#17-0756, GE Healthcare, Boston, MA, USA) at 4 °C for 2 h. Next, samples were washed ten times with 0.01 M PBS (containing 1% triton) before being boiled with 5× loading buffer for 10 min. For SDS-PAGE and Western blot analyses, the samples prepared and boiled with 5× loading buffer, as described above, were separated on SDS-PAGE before being transferred onto PVDF membranes (Cat# R0NB30936, Millipore, Billerica, MA, USA) using a Mini TransBlot cell wet transfer system (Model#1658030, Bio-Rad, Richmond, CA, USA). ..

    SDS Page:

    Article Title: Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid Shrimp.
    Article Snippet: .. For GST pull-down analysis, purified recombinant proteins (i.e., GST-PvBip, PvHMC, and GST) were incubated with Glutathione Sepharose 4B beads (Cat#17-0756, GE Healthcare, Boston, MA, USA) at 4 ◦C for 2 h. Next, samples were washed ten times with 0.01 M PBS (containing 1% triton) before being boiled with 5× loading buffer for 10 min. For SDS-PAGE and Western blot analyses, the samples prepared and boiled with 5× loading buffer, as described above, were separated on SDS-PAGE before being transferred onto PVDF membranes (Cat# R0NB30936, Millipore, Billerica, MA, USA) using a Mini TransBlot cell wet transfer system (Model#1658030, Bio-Rad, Richmond, CA, USA). ..

    Article Title: Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid Shrimp
    Article Snippet: .. For GST pull-down analysis, purified recombinant proteins (i.e., GST-PvBip, PvHMC, and GST) were incubated with Glutathione Sepharose 4B beads (Cat#17-0756, GE Healthcare, Boston, MA, USA) at 4 °C for 2 h. Next, samples were washed ten times with 0.01 M PBS (containing 1% triton) before being boiled with 5× loading buffer for 10 min. For SDS-PAGE and Western blot analyses, the samples prepared and boiled with 5× loading buffer, as described above, were separated on SDS-PAGE before being transferred onto PVDF membranes (Cat# R0NB30936, Millipore, Billerica, MA, USA) using a Mini TransBlot cell wet transfer system (Model#1658030, Bio-Rad, Richmond, CA, USA). ..

    Western Blot:

    Article Title: Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid Shrimp.
    Article Snippet: .. For GST pull-down analysis, purified recombinant proteins (i.e., GST-PvBip, PvHMC, and GST) were incubated with Glutathione Sepharose 4B beads (Cat#17-0756, GE Healthcare, Boston, MA, USA) at 4 ◦C for 2 h. Next, samples were washed ten times with 0.01 M PBS (containing 1% triton) before being boiled with 5× loading buffer for 10 min. For SDS-PAGE and Western blot analyses, the samples prepared and boiled with 5× loading buffer, as described above, were separated on SDS-PAGE before being transferred onto PVDF membranes (Cat# R0NB30936, Millipore, Billerica, MA, USA) using a Mini TransBlot cell wet transfer system (Model#1658030, Bio-Rad, Richmond, CA, USA). ..

    Article Title: Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid Shrimp
    Article Snippet: .. For GST pull-down analysis, purified recombinant proteins (i.e., GST-PvBip, PvHMC, and GST) were incubated with Glutathione Sepharose 4B beads (Cat#17-0756, GE Healthcare, Boston, MA, USA) at 4 °C for 2 h. Next, samples were washed ten times with 0.01 M PBS (containing 1% triton) before being boiled with 5× loading buffer for 10 min. For SDS-PAGE and Western blot analyses, the samples prepared and boiled with 5× loading buffer, as described above, were separated on SDS-PAGE before being transferred onto PVDF membranes (Cat# R0NB30936, Millipore, Billerica, MA, USA) using a Mini TransBlot cell wet transfer system (Model#1658030, Bio-Rad, Richmond, CA, USA). ..

    Membrane:

    Article Title: Wiskott Aldrich syndrome protein regulates non-selective autophagy and mitochondrial homeostasis in human myeloid cells
    Article Snippet: Protein lysates were reduced with DTT (Thermo Fisher Scientific), heat blocked and separated by SDS-PAGE using 4-12% pre-cast BisTris gels (Thermo Fisher Scientific) with MES running buffer (Thermo Fisher Scientific), using NuPAGE (Invitrogen) system. .. Resolved proteins were transferred to methanol-activated 0.2μm PVDF membrane (BioRad), using BioRad Mini Transblot Cell wet transfer system (100V for 1 hour) and transfer buffer (Thermo Fisher Scientific) with 20% methanol. .. After blocking in 5% skimmed milk for 1 hour at room temperature, membranes were incubated with primary antibodies [LAMP-1 (1:1000, BD Biosciences), LC3B (1:1000, Sigma) and WASp (1:500, BD Biosciences) as needed, with GAPDH (1:1000, Santa Cruz) as loading control] for either 1 hour at room temperature or overnight at 4°C.



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